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dc.creatorCoello,Christian Yanelly
dc.creatorMiceli,Clara Luz
dc.creatorOrantes,Carolina
dc.creatorDenDooven,Luc
dc.creatorGutiérrez,Federico Antonio
dc.date2010-01-01
dc.date.accessioned2020-06-09T15:24:17Z
dc.date.available2020-06-09T15:24:17Z
dc.identifierhttps://scielo.conicyt.cl/scielo.php?script=sci_arttext&pid=S0717-66432010000100003
dc.identifier.urihttps://bibliotecadigital.infor.cl/handle/20.500.12220/29586
dc.descriptionAn in vitro culture procedure was developed to induce shoots and roots of Guarianthe skinneri (Orchidaceae) plantlets regenerated from seed-derived protocorms on a Murashige and Skoog (MS) médium supplemented with 6-benzyladenine (BA), indole-3-acetic acid (IAA), a-naftalenacetic acid (NAA) and gibberellic acid (GA3). A máximum of 10.6 shoots was obtained with 16.1 μM NAA, 17.1 μM IAA, 6.3 x 10-9 μM GA3 and 0.0023 μM BA. A máximum of 4.0 roots on each shoot with 5.4 μM NAA, 17.1 μM IAA, 0.001 μM AG3 and 4.6 x 10-9 μM BA. Máximum shoot and root length was obtained with a minimum of GA3, but a máximum IAA. GA3, was the main factor controlling shoot and root induction and elongation.
dc.formattext/html
dc.languageen
dc.publisherFacultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción
dc.relation10.4067/S0717-66432010000100003
dc.rightsinfo:eu-repo/semantics/openAccess
dc.sourceGayana. Botánica v.67 n.1 2010
dc.subject6-benzyladenine
dc.subjectindole-3-acetic acid
dc.subjecta-naftalenacetic acid
dc.subjectgibberellic acid
dc.subjectshoot induction
dc.subjectrooting
dc.titlePlant growth regulators optimization for in vitro cultivation of the orchid Guarianthe skinneri (Bateman) Dressier & W.E.Higgins
dc.typeArtículo de revista


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